Ethylene, a key phytohormone, plays a vital role in the ripening of climacteric fruits like bananas, with its biosynthesis being a focal point of agricultural research due to its impact on fruit quality and shelf life. Transcription factors(TFs), such as MADS and NAC, can regulate ACC synthase (ACS) and ACC oxidase (ACO), which are crucial enzymes in ethylene synthesis, to control fruit ripening.