Extracellular vesicles (EVs) are cell-secreted lipid bilayer bioparticles with a diameter of 30 to 250 nm. They are a promising source of biomarkers for liquid biopsies for early cancer diagnosis and real-time monitoring of tumor development. However, analysis of nanosized EVs in blood samples has been hampered by the lack of effective, rapid, and standardized methods to isolate and purify EVs.