Mammalian cells are inherently complex due to subcellular compartments, thereby making the process of robust transcript targeting of nucleic acids somewhat challenging in the molecular biology lab. In a recent report now published in Nature Biotechnology, David Colognori and a research team headed by Chemistry Nobel Laureate Jennifer Doudna, who discovered and expanded on the CRISPR-Cas9 technology alongside Emmanuel Charpentier, in the year 2020, incorporated a new complex to the CRISPR complex during this study.