Baker's yeast, Saccharomyces cerevisiae, has been extensively engineered to produce a vast variety of chemicals that are not naturally produced by yeast, including the important monomer for synthetic rubber, isoprene. However, all organisms are naturally evolved for better survival rather than maximized biosynthesis of products of human interest. S. cerevisiae is not an exception. Impaired cell growth was observed during its engineering by pathway compartmentation for improved isoprene biosynthesis.