Interference leads to inaccurate Raman spectroscopic analysis of vitamin B12
Many natural products are complicated organic molecules. Despite this complexity, scientists are usually able to investigate them using spectroscopic techniques. However, a team of researchers has now discovered that care should be taken using Raman spectroscopy to analyze certain chiral molecules (molecules that have handedness; i.e., they can exist in two "mirror image" forms of each other). The study, published in the journal Angewandte Chemie, shows that interference with circularly polarized light can falsify results.