The Bolson tortoise (Bolson Gopherus flavomarginatus) is the largest land reptile in North America. It lives mainly in dry areas, in particular, in the Chihuahua desert in northern Mexico. In recent decades, its numbers have fallen by 50%, driving the International Union for Conservation of Nature to include it on its red list and it has been classified as endangered. Land tortoises are prone to suffering from a wide range of diseases that can deplete their numbers even more, therefore understanding potential pathogenic organisms could help in advancing conservation strategies.